For 2 h. Alternatively, the cell Macitentan lysates were incubated in 30 L anti-FLAG
For 2 h. Alternatively, the cell lysates were incubated in 30 L anti-FLAG affinity agarose gel (50 slurry) at 4 overnight. Immunoprecipitates were washed with ice-cold RIPA buffer (400 L)…